Integration of Dark Fermentation with Microbial Electrolysis Cells for Biohydrogen and Methane Production from Distillery Wastewater and Glycerol Waste Co-Digestion

This study aimed to enhance dark fermentative hydrogen production from co-digestion of distillery wastewater (DW) and glycerol waste (GW) through integration with microbial electrolysis cells. First, the optimal proportion DW GW in was investigated batch mode. The results show that at a ratio 99:1 (% v/v) gave highest yield 149.5 mL-H2/g − VSadded. Continuous using conducted continuously stirred tank reactor. As result, maximal 99.7 VSadded achieved, dominant hydrogen-producing bacterium Clostridium sensu stricto 7. fermentation effluent reactor later used produce methane MECs. maximum 115.1 mL-CH4/g obtained under an applied voltage 1 V continuous stirring 120–140 rpm. Microbial community analysis revealed Metahnobacterium, Methanomethylovorans, Methanoculleus, Methanosarcina were methanogenic archaea cell